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Hepatic NF-κB activity was determined by measuring both NF-κB-DNA-binding activity using nuclear proteins and the expression of NF-κB target genes

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Higher TMAO levels were associated with increased risk of all-cause death in CKD patients from mild to end-stage, which remained significance after controlling for glomerular filtration rates and other covariates, suggesting that TMAO can be an independent predictor for mortality in CKD patients ( 2.2 The mechanisms of trimethylamine N-oxide promoting the development of chronic kidney disease Inflammation is an important mechanism for the TMAO-mediated occurrence and development of CKD